产品货号：
Z11116
中文名称：
AQP2抗体
英文名称：
Anti-AQP2 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-AQP2 Antibody |
| 指标别名 | ADH water channel;AQP 2;AQP CD;AQP2;Aquaporin 2;aquaporin 2 (collecting duct);Aquaporin CD;WCH CD |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,IHC-F |
| 基因名称 | AQP2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2(245-260aa DWEEREVRRRQSVELH),identical to the related rat and mouse sequences. |
| 实际分子量 | 26～37KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | AQP2(Aquaporin 2) also called AQUAPORIN-CD,is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell.The AQP2 gene is mapped to chromosome 12q13,very close to the site of major intrinsic protein by situ hybridization.The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus.The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel.Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2,AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide(NO).The NO donors sodium nitroprusside(SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin(VP),stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane.SNP increased intracellular cGMP rather than cAMP,and exogenous cGMP stimulated AQP2 membrane insertion.Atrial natriuretic factor,which signals via cGMP,also stimulated AQP2 translocation.AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance.The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells. |
| Uniprot ID | P41181 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
| 基因名全称 | aquaporin 2(collecting duct) |
| 蛋白名全称 | Aquaporin-2(AQP-2) |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Immunohistochemistry in paraffin section (IHC) 1:50～400 Immunohistochemistry in frozen section (IHC-F) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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